Analysis Of Adipose Derived Induced Pluripotent Stem Cells

â€Å"Research Proposal† Medical Biotechnology, Health Sciences Honours Research Proposal 30/06/2015 John Drummond 2040768 1) Grant titles Scientific title Analysis of adipose derived induced pluripotent stem cells (iPSC’s) microRNA (miRNA) expression profile over multiple passages and pancreatic ÃŽ ²-cell miRNA expression profile post differentiation from iPSC’s. Simplified title Improvement of stem cell differentiation to pancreatic ÃŽ ²-cell for transplantation into diabetics. 2) Project summary Ethical concerns regarding the use of embryo-derived embryonic stem cells (ES), and the immune response elicited by differentiated ES cells, are being circumvented by using induced pluripotential stem cells (iPSCs) derived from patients’ somatic cells. These can be differentiated into other cell types including pancreatic ï  ¢-cells for diabetics. Unfortunately aspects of iPSC DNA regulation of differentiation impair the production of cells for transplant. Understanding the role of micro RNAs in iPSC differentiation could improve the therapeutic application of iPSCs. 3) Keywords Induced pluripotent stem cells (iPSC’s) Embryonic stem cells (ES) Micro RNA (miRNA) Epigenetics Pancreatic ÃŽ ²-cells 4) Clearance requirements: List any ethical implications approvals required. Describe each that applies to your proposal (ie. humans, animals, hazardous chemicals, biohazards, OGTR) in ~2 lines. Human research ethics committee (HREC) application and approval required to collectShow MoreRelatedRole Of The Wnt / ÃŽÂ’ On The Treatment Of Acute Respiratory Distress Syndrome ( Ards )1240 Words   |  5 PagesMany studies have been done regarding stem cells. One study looked at the role of the Wnt/ÃŽ ²-catenin signaling pathway in the differentiation of mesenchymal stem cells into type II alveolar epithelial cells for the treatment of acute respiratory distress syndrome (ARDS). This study was done in mice with acute respiratory distress syndrome. ÃŽ ²-catenin or GFP control were transfected into mice mesenchymal stem cells, and these mesenchymal stem cells were put into the trachea tissue of the mice. SeveralRead MoreAn Introduction Of Regenerative Medicine2246 Words   |  9 Pagesrelative modern and multidisciplinary field of study, encompassing molecular biology, genetics, immunology and biochemistry.[1] It aims to recover the normal function of dysfunctional tissue and organs through repairing, replacing or regenerating human cells, tissue or organs. [2,3] Generally, there are three interrelated aspects to the regenerative medicine scope, i.e. rejuvenation, replacement and regeneration, respectively.[4] It is also represented as the â€Å"R3† regenerative medicine paradigm for theRead MoreStem Cells And Its Effect On The Body During The Initial Process Of Growth And Development2750 Words   |  11 PagesStem cells develop itself into various types of cells inside the body during the initial process of growth and development. Coupled with it, stem cells also play the role of an internal recovery system, in which it replicates itself limitlessly to stocks up the rest of the cells till the individual remains alive. When a stem cell multiplies itself, every new cell produced has the ability to perform the functions of a stem cell or may become some other sort of cell with greater and better well versed